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The stability in protoplasts of all the mutant RNA constructs in protoplasts was found to be unchanged as compared to the wild-type constructs, independent of its translation efficiency not shown.

The complete plasmids were PCR-amplified using Pyrobest polymerase Takara with primers containing the mutation. They were digested with DpnI to select for the mutant plasmids Sambrook and Russell, The amplified fragment was sequenced.

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The mutations were stable in the progeny. This sequence conservation supports the secondary structure model.

The highly accessible regions forming the loops L of stem-loops SL 1—3 are marked on the right side of the gel. Color-coded bases indicate the levels of BzCN modification, with warmer colors indicating greater modification inset.

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Arrows indicate other variable positions in an alignment of MNSV sequences; arrows concentrate in unpaired regions. A Relative luciferase activity corresponding to the in vivo translation efficiency in melon protoplasts of the different constructs horizontal bars; obtained with capped RNA in black and uncapped RNA in gray corresponding to at least four independent experiments error bars indicate the standard deviation.

We studied the effects of these mutations on the in vivo cap-independent translation efficiency. Error bars represent the standard deviation of at least four independent experiments. Prediction of Additional RNA Interactions Between the Ends of MNSV Genomes We used the Transat bioinformatics tool, that detects conserved helices of high statistical significance, including pseudo-knotted, transient and alternative structures starting with a multiple sequence alignment Wiebe and Meyer, , to predict interactions between the ends of MNSV genomes.

Additionally, the tool also predicted an interaction between the same nucleotides of the Ma5TE and the six nucleotides located in ORF1, U—A red arrows.

But the possibility that these predicted interactions are transient and dynamic cannot be excluded. A reference shall be made in the inspection certificate to the verification of the uniformity over the strip length and the approval by the relevant third party.

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Close this window and log in. An approval procedure e. In cases of doubt or dispute, the latest edition of the German text only is valid.

Promoting, selling, recruiting, coursework and thesis posting is forbidden. BTW, the quote from Wiki is specifically referring to pressure vessels. Resources Digital Transformation may be defined in a number of different ways by analysts or enterprise software vendors.

Bta-miR attenuates the cell apoptosis of Madin—Darby bovine kidney cells through directly targeting caspase 7 The cell apoptosis of bladder cancer cells could be regulated by miR through targeting caspase 2 Consistent with its function in other cancers, CASC2 knockdown significantly promoted tumor cell viability, suppressed tumor cell apoptosis, and increased the resistance of hepatocellular carcinoma cell to TRAIL.

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CASC2 expression is down-regulated in the primary tumor, and more down-regulated in advanced stages; higher CASC2 expression causes slight up-regulation in the survival of patients with hepatocellular carcinoma, while lower miR and miR expression is correlated with longer survival of patients with hepatocellular carcinoma.

We used expression of RNU6B as an endogenous control. OD nm value was measured.

Briefly, the cell samples were harvested with 0. Each experiment was repeated three times in triplicate. Western blot analysis The protein levels of caspase-3, cleaved-caspase-3, caspase-8, and cleaved-caspase-8 in hepatocellular carcinoma cells were detected by performing immunoblotting. GAPDH was used as an endogenous protein for normalization.

After cultured overnight, cells were co-transfected with the indicated vectors and miRNA mimics or miRNA inhibitor, respectively.

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References 1. Waller, L.

Hepatocellular carcinoma: a comprehensive review. World J.

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